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GENERAL
TITLE
CRYSTAL STRUCTURE OF A SELENIUM-DERIVATIZED COMPLEX OF THE BACTERIAL CELLULOSE SECRETION REGULATORS BCSR AND BCSQ, CRYSTALLIZED IN THE PRESENCE OF ADP
DATE LAST MODIFIED
-
RELEASE
24 Feb 2021
OBSOLETE
CLASSIFICATION
SIGNALING PROTEIN
EXPERIMENT TYPE
X-RAY DIFFRACTION
SPACE GROUP
P 1 21 1
RESOLUTION RANGE HIGH
2.09
RESOLUTION RANGE LOW
50
PUBMED ID
DOI
10.1126/SCIADV.ABD8049

Chains

Structure ChainTarget ProteinGeneExpression SystemOrganismSynonymsActions
P0DP92 BCSQ, YHJQ, A8C65_00290, ACU57_05335, BMT91_17060, ESCHERICHIA COLI BL21(DE3) ESCHERICHIA COLI CELLULOSE BIOSYNTHESIS PROTEIN BCSQ,CELLULOSE SYNTHASEOPERON PROTEIN YHJQ,CELLULOSE SYNTHASE,PUTATIVE,BACTERIAL CELLULOSESYNTHESIS SUBUNIT QREGION CORRESPONDING TO THE BCSRQ TANDEM WAS CLONED INTO THE PET21BEXPRESSION VECTOR WITH A C-TERMINAL HEXAHISTIDINE TAG ON BCSQ. THEPURIFIED SELENIUM DERIVATED BCSRQ COMPLEX WAS TREATED WITH EDTA ANDINCUBATED WITH ADP PRIOR TO CRYSTALLISATION. ESCHERICHIA COLI
CELLULOSE BIOSYNTHESIS PROTEIN BCSQ,CELLULOSE SYNT ...
Mutations
P0DP92 BCSQ, YHJQ, A8C65_00290, ACU57_05335, BMT91_17060, ESCHERICHIA COLI BL21(DE3) ESCHERICHIA COLI CELLULOSE BIOSYNTHESIS PROTEIN BCSQ,CELLULOSE SYNTHASEOPERON PROTEIN YHJQ,CELLULOSE SYNTHASE,PUTATIVE,BACTERIAL CELLULOSESYNTHESIS SUBUNIT QREGION CORRESPONDING TO THE BCSRQ TANDEM WAS CLONED INTO THE PET21BEXPRESSION VECTOR WITH A C-TERMINAL HEXAHISTIDINE TAG ON BCSQ. THEPURIFIED SELENIUM DERIVATED BCSRQ COMPLEX WAS TREATED WITH EDTA ANDINCUBATED WITH ADP PRIOR TO CRYSTALLISATION. ESCHERICHIA COLI
CELLULOSE BIOSYNTHESIS PROTEIN BCSQ,CELLULOSE SYNT ...
Mutations
P0ADJ3 YHJR, A6V01_14365, A8C65_00295, A9R57_18690, AC789_1C39040, ESCHERICHIA COLI BL21(DE3) ESCHERICHIA COLI FIG004405: PUTATIVE CYTOPLASMIC PROTEIN,PROTEIN YHJR,PROTEINOF UNCHARACTERIZED FUNCTION (DUF2629),BACTERIAL CELLULOSE SYNTHESISSUBUNIT RREGION CORRESPONDING TO THE BCSRQ TANDEM WAS CLONED INTO THE PET21BEXPRESSION VECTOR WITH A C-TERMINAL HEXAHISTIDINE TAG ON BCSQ. THEPURIFIED SELENIUM DERIVATED BCSRQ COMPLEX WAS TREATED WITH EDTA ANDINCUBATED WITH ADP PRIOR TO CRYSTALLISATION. ESCHERICHIA COLI
FIG004405: PUTATIVE CYTOPLASMIC PROTEIN,PROTEIN YH ...
Mutations
P0ADJ3 YHJR, A6V01_14365, A8C65_00295, A9R57_18690, AC789_1C39040, ESCHERICHIA COLI BL21(DE3) ESCHERICHIA COLI FIG004405: PUTATIVE CYTOPLASMIC PROTEIN,PROTEIN YHJR,PROTEINOF UNCHARACTERIZED FUNCTION (DUF2629),BACTERIAL CELLULOSE SYNTHESISSUBUNIT RREGION CORRESPONDING TO THE BCSRQ TANDEM WAS CLONED INTO THE PET21BEXPRESSION VECTOR WITH A C-TERMINAL HEXAHISTIDINE TAG ON BCSQ. THEPURIFIED SELENIUM DERIVATED BCSRQ COMPLEX WAS TREATED WITH EDTA ANDINCUBATED WITH ADP PRIOR TO CRYSTALLISATION. ESCHERICHIA COLI
FIG004405: PUTATIVE CYTOPLASMIC PROTEIN,PROTEIN YH ...
Mutations
NGL Viewer is used for the molecular visualization.
  • AS Rose, AR Bradley, Y Valasatava, JM Duarte, A Prlić and PW Rose. NGL viewer: web-based molecular graphics for large complexes. Bioinformatics: bty419, 2018. doi:10.1093/bioinformatics/bty419
  • AS Rose and PW Hildebrand. NGL Viewer: a web application for molecular visualization. Nucl Acids Res (1 July 2015) 43 (W1): W576-W579 first published online April 29, 2015. doi:10.1093/nar/gkv402