6YBU

3D Structure View

CAVITIES

STRUCTURE SETTINGS

LIGANDS

Chain 6YBU_A

Chain 6YBU_B

Chain 6YBU_C

Chain 6YBU_D

Chain 6YBU_E

Chain 6YBU_F

Chain 6YBU_G

Chain 6YBU_H

Chain 6YBU_I

Chain 6YBU_J

Chain 6YBU_K

Chain 6YBU_L

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GENERAL

TITLE

CRYSTAL STRUCTURE OF A NATIVE BCSE (349-523) RQ COMPLEX WITH C-DI-GMP AND ATP BOUND

DATE LAST MODIFIED

RELEASE

24 Feb 2021

OBSOLETE

CLASSIFICATION

SIGNALING PROTEIN

EXPERIMENT TYPE

X-RAY DIFFRACTION

SPACE GROUP

P 1 21 1

RESOLUTION RANGE HIGH

2.49

RESOLUTION RANGE LOW

PUBMED ID

33563593

DOI

10.1126/SCIADV.ABD8049

PDBe

6YBU

LIGANDS

322120 , 2694373

Chains

Target Protein	Gene	Expression System	Organism	Synonyms	Actions
P0DP92	BCSQ, YHJQ, A8C65_00290, ACU57_05335, BMT91_17060,	ESCHERICHIA COLI BL21(DE3)	ESCHERICHIA COLI	CELLULOSE BIOSYNTHESIS PROTEIN BCSQ,CELLULOSE SYNTHASEOPERON PROTEIN YHJQ,CELLULOSE SYNTHASE,PUTATIVE, BACTERIAL CELLULOSESYNTHESIS SUBUNIT Q	ESCHERICHIA COLI	CELLULOSE BIOSYNTHESIS PROTEIN BCSQ,CELLULOSE SYNT ...	Mutations
P0DP92	BCSQ, YHJQ, A8C65_00290, ACU57_05335, BMT91_17060,	ESCHERICHIA COLI BL21(DE3)	ESCHERICHIA COLI	CELLULOSE BIOSYNTHESIS PROTEIN BCSQ,CELLULOSE SYNTHASEOPERON PROTEIN YHJQ,CELLULOSE SYNTHASE,PUTATIVE, BACTERIAL CELLULOSESYNTHESIS SUBUNIT Q	ESCHERICHIA COLI	CELLULOSE BIOSYNTHESIS PROTEIN BCSQ,CELLULOSE SYNT ...	Mutations
P0ADJ3	YHJR, A6V01_14365, A8C65_00295, A9R57_18690, AC789_1C39040,	ESCHERICHIA COLI BL21(DE3)	ESCHERICHIA COLI	FIG004405: PUTATIVE CYTOPLASMIC PROTEIN,PROTEIN YHJR,PROTEINOF UNCHARACTERIZED FUNCTION (DUF2629), BACTERIAL CELLULOSE SYNTHESISSUBUNIT RWAS CLONED INTO THE PPROEX-HTB WITH A CLEAVABLE N-TERMINALHEXAHISTIDINE TAG TO BCSR. THE SAMPLE WERE PURIFIED AND CRYSTALLISEDWITHOUT THE HIS-TAG. THE GPMGS CORRESPOND TO THE NTER ADDITIONALRESIDUES REMAINING AFTER CLEAVAGE. BACTERIAL CELLULOSE SYNTHESISSUBUNIT R.	ESCHERICHIA COLI	FIG004405: PUTATIVE CYTOPLASMIC PROTEIN,PROTEIN YH ...	Mutations
P0ADJ3	YHJR, A6V01_14365, A8C65_00295, A9R57_18690, AC789_1C39040,	ESCHERICHIA COLI BL21(DE3)	ESCHERICHIA COLI	FIG004405: PUTATIVE CYTOPLASMIC PROTEIN,PROTEIN YHJR,PROTEINOF UNCHARACTERIZED FUNCTION (DUF2629), BACTERIAL CELLULOSE SYNTHESISSUBUNIT RWAS CLONED INTO THE PPROEX-HTB WITH A CLEAVABLE N-TERMINALHEXAHISTIDINE TAG TO BCSR. THE SAMPLE WERE PURIFIED AND CRYSTALLISEDWITHOUT THE HIS-TAG. THE GPMGS CORRESPOND TO THE NTER ADDITIONALRESIDUES REMAINING AFTER CLEAVAGE. BACTERIAL CELLULOSE SYNTHESISSUBUNIT R.	ESCHERICHIA COLI	FIG004405: PUTATIVE CYTOPLASMIC PROTEIN,PROTEIN YH ...	Mutations
P37657	BCSE, YHJS, B3536, JW3504	ESCHERICHIA COLI BL21(DE3)	ESCHERICHIA COLI (STRAIN K12)	CELLULOSE BIOSYNTHESIS PROTEIN BCSE, BACTERIAL CELLULOSESYNTHESIS SUBUNIT ETO 523 WAS CLONED INTO A MODIFIED PRSF-DUET TO EXPRESS THE TRUNCATEDPROTEIN WITHOUT A TAG. THE FIRST ADDITIONAL MGSM RESIDUES COME FROMTHE CLONING. BACTERIAL CELLULOSE SYNTHESIS SUBUNIT E.	ESCHERICHIA COLI (STRAIN K12)	CELLULOSE BIOSYNTHESIS PROTEIN BCSE, BACTERIAL CEL ...	Mutations
P37657	BCSE, YHJS, B3536, JW3504	ESCHERICHIA COLI BL21(DE3)	ESCHERICHIA COLI (STRAIN K12)	CELLULOSE BIOSYNTHESIS PROTEIN BCSE, BACTERIAL CELLULOSESYNTHESIS SUBUNIT ETO 523 WAS CLONED INTO A MODIFIED PRSF-DUET TO EXPRESS THE TRUNCATEDPROTEIN WITHOUT A TAG. THE FIRST ADDITIONAL MGSM RESIDUES COME FROMTHE CLONING. BACTERIAL CELLULOSE SYNTHESIS SUBUNIT E.	ESCHERICHIA COLI (STRAIN K12)	CELLULOSE BIOSYNTHESIS PROTEIN BCSE, BACTERIAL CEL ...	Mutations
P0DP92	BCSQ, YHJQ, A8C65_00290, ACU57_05335, BMT91_17060,	ESCHERICHIA COLI BL21(DE3)	ESCHERICHIA COLI	CELLULOSE BIOSYNTHESIS PROTEIN BCSQ,CELLULOSE SYNTHASEOPERON PROTEIN YHJQ,CELLULOSE SYNTHASE,PUTATIVE, BACTERIAL CELLULOSESYNTHESIS SUBUNIT Q	ESCHERICHIA COLI	CELLULOSE BIOSYNTHESIS PROTEIN BCSQ,CELLULOSE SYNT ...	Mutations
P0DP92	BCSQ, YHJQ, A8C65_00290, ACU57_05335, BMT91_17060,	ESCHERICHIA COLI BL21(DE3)	ESCHERICHIA COLI	CELLULOSE BIOSYNTHESIS PROTEIN BCSQ,CELLULOSE SYNTHASEOPERON PROTEIN YHJQ,CELLULOSE SYNTHASE,PUTATIVE, BACTERIAL CELLULOSESYNTHESIS SUBUNIT Q	ESCHERICHIA COLI	CELLULOSE BIOSYNTHESIS PROTEIN BCSQ,CELLULOSE SYNT ...	Mutations
P0ADJ3	YHJR, A6V01_14365, A8C65_00295, A9R57_18690, AC789_1C39040,	ESCHERICHIA COLI BL21(DE3)	ESCHERICHIA COLI	FIG004405: PUTATIVE CYTOPLASMIC PROTEIN,PROTEIN YHJR,PROTEINOF UNCHARACTERIZED FUNCTION (DUF2629), BACTERIAL CELLULOSE SYNTHESISSUBUNIT RWAS CLONED INTO THE PPROEX-HTB WITH A CLEAVABLE N-TERMINALHEXAHISTIDINE TAG TO BCSR. THE SAMPLE WERE PURIFIED AND CRYSTALLISEDWITHOUT THE HIS-TAG. THE GPMGS CORRESPOND TO THE NTER ADDITIONALRESIDUES REMAINING AFTER CLEAVAGE. BACTERIAL CELLULOSE SYNTHESISSUBUNIT R.	ESCHERICHIA COLI	FIG004405: PUTATIVE CYTOPLASMIC PROTEIN,PROTEIN YH ...	Mutations
P0ADJ3	YHJR, A6V01_14365, A8C65_00295, A9R57_18690, AC789_1C39040,	ESCHERICHIA COLI BL21(DE3)	ESCHERICHIA COLI	FIG004405: PUTATIVE CYTOPLASMIC PROTEIN,PROTEIN YHJR,PROTEINOF UNCHARACTERIZED FUNCTION (DUF2629), BACTERIAL CELLULOSE SYNTHESISSUBUNIT RWAS CLONED INTO THE PPROEX-HTB WITH A CLEAVABLE N-TERMINALHEXAHISTIDINE TAG TO BCSR. THE SAMPLE WERE PURIFIED AND CRYSTALLISEDWITHOUT THE HIS-TAG. THE GPMGS CORRESPOND TO THE NTER ADDITIONALRESIDUES REMAINING AFTER CLEAVAGE. BACTERIAL CELLULOSE SYNTHESISSUBUNIT R.	ESCHERICHIA COLI	FIG004405: PUTATIVE CYTOPLASMIC PROTEIN,PROTEIN YH ...	Mutations

NGL Viewer is used for the molecular visualization.

AS Rose, AR Bradley, Y Valasatava, JM Duarte, A Prlić and PW Rose. NGL viewer: web-based molecular graphics for large complexes. Bioinformatics: bty419, 2018. doi:10.1093/bioinformatics/bty419
AS Rose and PW Hildebrand. NGL Viewer: a web application for molecular visualization. Nucl Acids Res (1 July 2015) 43 (W1): W576-W579 first published online April 29, 2015. doi:10.1093/nar/gkv402