FEATURES

FAMILY

DESCRIPTION

Also known as PATF_PENEN, patF. Part of the gene cluster that mediates the biosynthesis of patulin, an acetate-derived tetraketide mycotoxin produced by several fungal species that shows antimicrobial properties against several bacteria (PubMed:25120234, PubMed:30100914, PubMed:25625822, PubMed:30680886). The pathway begins with the synthesis of 6-methylsalicylic acid by the polyketide synthase (PKS) patK via condensation of acetate and malonate units (PubMed:30680886). The 6-methylsalicylic acid decarboxylase patG then catalyzes the decarboxylation of 6-methylsalicylic acid to yield m-cresol (also known as 3-methylphenol) (PubMed:30680886). These first reactions occur in the cytosol (PubMed:30680886). The intermediate m-cresol is then transported into the endoplasmic reticulum where the cytochrome P450 monooxygenase patH converts it to m-hydroxybenzyl alcohol, which is further converted to gentisyl alcohol by the cytochrome P450 monooxygenase patI (PubMed:30680886). The oxidoreductases patJ and patO further convert gentisyl alcohol to isoepoxydon in the vacuole (PubMed:30680886). PatN catalyzes then the transformation of isoepoxydon into phyllostine (PubMed:30680886). The cluster protein patF is responsible for the conversion from phyllostine to neopatulin whereas the alcohol dehydrogenase patD converts neopatulin to E-ascladiol (PubMed:30680886). The steps between isoepoxydon and E-ascladiol occur in the cytosol, and E-ascladiol is probably secreted to the extracellular space by one of the cluster-specific transporters patC or patM (PubMed:30680886). Finally, the secreted patulin synthase patE catalyzes the conversion of E-ascladiol to patulin (PubMed:30680886).

EXTERNAL LINKS

patF

Patulin biosynthesis cluster protein F

patF

Molecular Synopsis