FEATURES

FAMILY

DESCRIPTION

Also known as QUEFL_PYRCJ, queF-L. Is responsible for the final step in the biosynthesis of archaeosine, a modified nucleoside present in the dihydrouridine loop (D-loop) of archaeal tRNA (PubMed:28383498, PubMed:22032275). Catalyzes the conversion of 7-cyano-7-deazaguanine (preQ0)-modified tRNA to archaeosine-tRNA, transforming a nitrile group to a formamidine group. Can use neither glutamine nor asparagine as amino donor in vitro, is only able to utilize free ammonium (PubMed:28383498). However, the enzyme might function in vivo with a partner that serves to generate ammonium. Forms a symmetric tunnel-fold (T-fold) homodecamer of two head-to-head facing pentameric subunits, with 10 active sites at the intermonomer interfaces.

EXTERNAL LINKS

queF-L

Archaeosine synthase

queF-L

Molecular Synopsis