FEATURES

FAMILY

DESCRIPTION

Also known as TOP6B_SACSH, top6B. Relaxes both positive and negative supercoils and exhibits a strong decatenase and unknotting activity; it cannot introduce DNA supercoils (PubMed:7961685). ATP is absolutely required for DNA cleavage; the nonhydrolyzable analog AMP-PNP generates nicked or linear products from a supercoiled dsDNA substrate. Generates staggered two-nucleotide long 5' overhangs. The enzyme is covalently attached transiently to the 5'-ends of the cleaved strands (PubMed:11485995). Homodimer. Heterotetramer of two Top6A and two Top6B chains.

EXTERNAL LINKS

uniprot
enzyme classification

top6B

Mutations

Mutation(s) distribution

Mutations table