FEATURES

FAMILY

DESCRIPTION

Also known as REPA_BPPHS, A. Plays an essential role in viral DNA replication. Binds the origin of replication and cleaves the dsDNA replicative form I (RFI) and becomes covalently bound to it via phosphotyrosine bond, generating the dsDNA replicative form II (RFII). In turn, viral DNA replication initiates in the presence of host Rep and DNA polymerase III at the 3'-OH of the cleavage site. After one round of rolling circle synthesis, protein A is linked to the newly synthesized ssDNA and joins the ends of the displaced strand to generate a circular single-stranded molecule ready to be packed into a virion., The A* protein binds to double-stranded DNA and prevents hydrolysis by nucleases. Additionally, A* is an inhibitor of DNA replication and may have a role in the transition from semiconservative replicative form DNA replication to single-stranded DNA synthesis in the life cycle.

EXTERNAL LINKS

A

Replication-associated protein A

A

Molecular Synopsis