FEATURES

FAMILY

DESCRIPTION

Also known as GLNK_ECOLI, glnK, ybaI. Involved in the regulation of nitrogen metabolism (PubMed:8843440, PubMed:10760266, PubMed:11847102, PubMed:12366843, PubMed:14668330, PubMed:28538158). Regulates the activity of its targets by protein-protein interaction in response to the nitrogen status of the cell (PubMed:8843440, PubMed:10760266, PubMed:11847102, PubMed:14668330). Involved in the regulation of the ammonium transporter AmtB so as to optimize ammonium uptake under all growth conditions (PubMed:11847102, PubMed:14668330, PubMed:16864585). In nitrogen-limited conditions, GlnK does not interact with AmtB, which remains active and imports ammonium. When extracellular ammonium increases, GlnK associates tightly with AmtB in the inner membrane, thereby inhibiting the transporter activity (PubMed:11847102, PubMed:14668330, PubMed:16864585). Also involved in the regulation of the glutamine synthetase adenylyltransferase/adenylyl-removing (AT/AR) enzyme GlnE and the glutamine synthetase GlnA (PubMed:8843440, PubMed:10760266, PubMed:28538158). In nitrogen-limited conditions, formation of uridylylated GlnB(PII)/GlnK heterotrimers may fine-regulate the activation of GlnA: uridylylated heterotrimers stimulate the deadenylation and the activation of GlnA whereas uridylylated GlnK homotrimers do not stimulate, or hardly stimulate, the deadenylation of GlnA (PubMed:10760266). In addition, regulates the expression of Ntr genes during nitrogen starvation, probably via the control of the levels of phosphorylated NRI during different stages of the response to nitrogen limitation (PubMed:12366843). Homotrimer (PubMed:10760266, PubMed:9733647, PubMed:17220269). In response to elevation of the extracellular ammonium concentration, interacts and forms a complex with AmtB (PubMed:11847102, PubMed:14668330, PubMed:17220269, PubMed:17190799, PubMed:16864585, PubMed:20639578). Binds to the cytoplasmic face of AmtB with a stoichiometry of AmtB(3):GlnK(3) (PubMed:17220269, PubMed:17190799). Sequestration by AmtB is reversible (PubMed:14668330). In nitrogen-poor medium, can form heterotrimers with GlnB (PII protein) (PubMed:10760266).

Also known as GLNK_ECOLI, glnK, ybaI. Involved in the regulation of nitrogen metabolism (PubMed:8843440, PubMed:10760266, PubMed:11847102, PubMed:12366843, PubMed:14668330, PubMed:28538158). Re ...

EXTERNAL LINKS

glnK

Nitrogen regulatory protein GlnK

glnK

Molecular Synopsis