FEATURES

FAMILY

DESCRIPTION

Also known as NUSA_ECOLI, nusA. Part of the processive rRNA transcription and antitermination complex (rrnTAC). The complex forms an RNA-chaperone ring around the RNA exit tunnel of RNA polymerase (RNAP). It supports rapid transcription and antitermination of rRNA operons, cotranscriptional rRNA folding, and annealing of distal rRNA regions to allow correct ribosome biogenesis (PubMed:32871103). Participates in both transcription termination and antitermination. Involved in a variety of cellular termination and antitermination processes, such as Rho-dependent transcriptional termination and intrinsic termination (PubMed:31020314). Domain AR2 interacts with a large number of other proteins and may serve as a platform to recruit these factors for transcriptional regulation (PubMed:31127279). Involved in phage lambda N-mediated transcriptional antitermination. Also important for coordinating the cellular responses to DNA damage by coupling the processes of nucleotide excision repair and translesion synthesis to transcription. Monomer. Binds directly to the core enzyme of the DNA-dependent RNA polymerase (RNAP) (PubMed:6263495, PubMed:1856861). Interacts with nascent RNA (PubMed:7536848). Interacts with the termination Rho factor (PubMed:6096352). Interacts with the phage lambda N protein (PubMed:6154941). Interacts with SuhB via the AR2 domain, crystallizes as a 2:1 SuhB:NusA heterotrimer. The rRNA transcription and antitermination complex (rrnTAC) consists of RNAP, NusA, NusB, NusE (rpsJ), NusG, SubB, ribosomal protein S4, DNA and precursor rRNA; S4 is more flexible than other subunits (PubMed:31020314, PubMed:31127279). NusG and the alpha-CTD of RNAP interact with the AR2 domain (PubMed:31127279).

EXTERNAL LINKS

nusA

Transcription termination/antitermination protein NusA

nusA

Molecular Synopsis