FEATURES

FAMILY

DESCRIPTION

Also known as LNT_ECOLI, lnt, cutE. Catalyzes the phospholipid dependent N-acylation of the N-terminal cysteine of apolipoprotein, the last step in lipoprotein maturation (PubMed:2032623, PubMed:21676878, PubMed:28885614, PubMed:28675161). Utilizes a two-step reaction via a ping-pong mechanism (PubMed:21676878, PubMed:28675161). Lnt undergoes covalent modification in the presence of phospholipids, resulting in a thioester acyl-enzyme intermediate. It then transfers the acyl chain to the amine group of the N-terminal diacylglyceryl-modified cysteine of apolipoprotein, leading to the formation of mature triacylated lipoprotein (PubMed:21676878, PubMed:28675161). In vitro, can utilize the phospholipids phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidic acid (PA) or cardiolipin (CL) (PubMed:2032623, PubMed:21676878). PE is the most efficient acyl donor (PubMed:21676878). Monomer.

EXTERNAL LINKS

lnt

Apolipoprotein N-acyltransferase

lnt

Molecular Synopsis