FEATURES

FAMILY

NtaA/SnaA/SoxA(DszA) monooxygenase family

DESCRIPTION

Also known as RUTA_ECOLI, rutA, ycdM. Catalyzes the pyrimidine ring opening between N-3 and C-4 by an unusual flavin hydroperoxide-catalyzed mechanism, adding oxygen atoms in the process to yield ureidoacrylate peracid, that immediately reacts with FMN forming ureidoacrylate and FMN-N(5)-oxide. The FMN-N(5)-oxide reacts spontaneously with NADH to produce FMN. Requires the flavin reductase RutF to regenerate FMN in vivo. RutF can be substituted by Fre in vitro.

EXTERNAL LINKS

uniprot
enzyme classification

rutA

Pyrimidine monooxygenase RutA

rutA

Molecular Synopsis