FEATURES

FAMILY

DESCRIPTION

Also known as TOP1_MYCTU, topA. Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex (PubMed:8921893, PubMed:20724443). Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone., The C-terminus (residues 622-934) inhibits RNA cleavage by MazF4. Monomer (By similarity). Interacts with mRNA interferase MazF4.

EXTERNAL LINKS

topA

Mutations

Mutation(s) distribution

Mutations table