FEATURES

FAMILY

filoviridae transcriptional activator VP30 family

DESCRIPTION

Also known as VP30_EBOZ5, VP30. Multifunctional protein that acts as a viral transcriptional activator. Promotes read-through of an RNA hairpin in the NP open reading frame to enhance viral transcription. Mechanistically, nonphosphorylated VP30 hexamers form a ternary complex with the viral leader RNA. Clamps the RNA template and the complex VP35-polymerase L together, thereby increasing the polymerase affinity for the RNA template to increase transcription initiation despite the presence of RNA secondary structures. Assists also stop-start transcription at gene junctions to promote transcription of downstream genes. Interaction with NP plays a critical role in transcription initiation by recognizing the RNA stem loop (By similarity). Interaction with host RBBP6 interferes with NP-VP30 interaction and inhibits viral RNA synthesis (PubMed:30550789). Also acts as a suppressor of RNA silencing by interacting with host DICER1 and TARBP2/TRBP (PubMed:21228243). Homohexamer; hexamerization is essential for RNA binding. Interacts with the nucleoprotein/NP; this interaction plays both essential and inhibitory roles in viral RNA synthesis. Interacts with VP35. Interacts with host STAU1 (By similarity). Interacts (via C-terminus) with host ubiquitin ligase RBBP6 isoform 1 (PubMed:30550789). Interacts with host DICER1; this interaction prevents TARBP2/TRBP binding to DICER1 and thus allows the virus to counteract host RNA silencing (PubMed:21228243). Interacts with host TARBP2/TRBP; this interaction, which occurs only in the presence of siRNA, prevents TARBP2 binding to DICER1 and thus allows the virus to counteract host RNA silencing (PubMed:21228243).

EXTERNAL LINKS

VP30

Mutations

Mutation(s) distribution

Mutations table