FEATURES

FAMILY

DESCRIPTION

Also known as ENDA_AERPE, endA. Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2', 3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp. Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.

EXTERNAL LINKS

uniprot
enzyme classification

endA

tRNA-splicing endonuclease

endA

Molecular Synopsis