FEATURES

FAMILY

DESCRIPTION

Also known as ASNO_STRCO, asnO. Catalyzes the 3-hydroxylation of L-asparagine to (2S, 3S)-3-hydroxyasparagine. The 3-hydroxylated asparagine produced is incorporated at position 9 during the biosynthesis of the non-ribosomally synthesized calcium-dependent antibiotic (CDA), a 11-residue acidic lipopeptide lactone. Is able to hydroxylate only free L-asparagine, since it hydroxylates neither a CDA analog with unmodified Asn at position 9 nor a peptidyl-carrier-protein (PCP)-bound asparagine. Is not active toward D-asparagine.

EXTERNAL LINKS

uniprot
enzyme classification

asnO

L-asparagine oxygenase

asnO

Molecular Synopsis