FEATURES

FAMILY

DESCRIPTION

Also known as H2018_AKKM8, Amuc_2018. Hydrolyzes terminal GlcNAc residues from terminally unbranched N-glycans and from chitobiose. Hydrolyzes beta-1, 6-linked N-acetylglucosamine and beta-1, 4-linked N-acetylgalactosamine from pNP-alpha-GalNAc[beta1, 3Gal]beta1, 6GlcNAc and pNP-beta-GlcNAc-beta1, 4-GalNAc substrates, respectively, as well as beta-1, 2-linked N-acetylglucosamine units from the non-reducing end of N-glycans. Hydrolyzes GlcNAc residues linked to alpha1, 3- or alpha1, 6-mannose branch, but has low activity on substrates with more than one GlcNAc residue on one of the mannose branches. Releases terminal GlcNAc moieties from the N-glycopeptide Gly-Glu-Asn-(GlcNAc2Man3GlcNAc2)-Arg with high efficiency. Has moderate hydrolytic activity on the chitobiose moiety of N-glycopeptide substrate Gly-Glu-Asn-(GlcNAc2)-Arg. Does not hydrolyze GlcNAc residues from N-glycan structures bearing a bisecting GlcNAc moiety (beta1, 4-linked GlcNAc to the beta1, 4-linked core mannose) (PubMed:29304441). Potentially capable of cleaving the specific glycoside linkages in the process of mucin degradation in human intestinal tract (Probable). Hydrolyzes synthetic substrate pNP-beta-GlcNAc with high activity and pNP-beta-GalNAc to a lesser extent (PubMed:29304441, PubMed:30846208). Does not hydrolyze pNP-beta-glucose, pNP-beta-galactose, pNP-alpha-glucose, pNP-alpha-galactose, pNP-alpha-GlcNAc or pNP-alpha-fucose (PubMed:29304441).

EXTERNAL LINKS

Amuc_2018

Beta-hexosaminidase Amuc_2018

Amuc_2018

Molecular Synopsis