FEATURES

FAMILY

DESCRIPTION

Also known as PRIM_BPT4, 61, 58. Synthesizes short RNA primers for the lagging strand DNA replication (PubMed:2422175, PubMed:3038901, PubMed:11735390, PubMed:22869700). The primase synthesizes short RNA primers on the lagging strand that the polymerase elongates using dNTPs (PubMed:2422175, PubMed:3038901). Recognizes two trinucleotide sequences 5'-GTT-3' and 5'-GCT-3' in vitro, but uses only the first as the priming site in vivo (PubMed:2422175). Monomer (PubMed:11735390). Hexamer (PubMed:11735390, PubMed:15897200, PubMed:15738414). Interacts with the DnaB-like replicative helicase; this interaction forms the active primosome complex, which is composed of 6 helicase and 1 primase subunits and expresses full helicase and primase activities (PubMed:22869700, PubMed:23578280, PubMed:11735390). Interacts (via C-terminus) with the single-stranded DNA-binding protein (PubMed:11735390). Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single-stranded DNA binding protein, the primase, the DnaB-like replicative helicase and the helicase assembly factor (PubMed:16800624).

Also known as PRIM_BPT4, 61, 58. Synthesizes short RNA primers for the lagging strand DNA replication (PubMed:2422175, PubMed:3038901, PubMed:11735390, PubMed:22869700). The primase synthesizes s ...

EXTERNAL LINKS

61

DNA primase

61

Molecular Synopsis