FEATURES

FAMILY

prokaryotic/mitochondrial release factor family

DESCRIPTION

Also known as RF2_ECOLI, prfB, supK. Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA (PubMed:11118225, PubMed:17932046). Acts as a peptidyl-tRNA hydrolase (PubMed:22857598, PubMed:27934701). In the presence of truncated mRNA in the 70S ribosome, ArfA and RF2 interact such that the GGQ peptide hydrolysis motif of RF2 rises into the peptidyl-transferase center and releases the ribosome (PubMed:27906160, PubMed:27906161, PubMed:27934701, PubMed:28077875). Recruited by ArfA to rescue stalled ribosomes in the absence of a normal stop codon (PubMed:22857598, PubMed:22922063, PubMed:25355516). Interacts with the ribosome (PubMed:22857598, PubMed:22922063, PubMed:25355516, PubMed:12511960, PubMed:12511961). Recruited to stalled ribosomes by ArfA, in the presence of truncated mRNA, ArfA influences RF2 conformation so RF2 can hydrolyze the peptidyl-tRNA bond (PubMed:27906160, PubMed:27906161, PubMed:27934701, PubMed:28077875).

EXTERNAL LINKS

prfB

Structural Ligandability

Domains Chains

Distribution