FEATURES

FAMILY

DESCRIPTION

Also known as ENTE_ECOLI, entE. Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2, 3-dihydroxybenzoyl)-serine. The serine trilactone serves as a scaffolding for the three catechol functionalities that provide hexadentate coordination for the tightly ligated iron(2+) atoms. EntE proccesses via a two-step adenylation-ligation reaction (bi-uni-uni-bi ping-pong mechanism). First, it catalyzes the activation of the carboxylate group of 2, 3-dihydroxy-benzoate (DHB), via a reversible ATP-dependent pyrophosphate exchange reactions to yield the acyladenylate intermediate 2, 3-dihydroxybenzoyl-AMP. It can also transfer AMP to salicylate, 2, 4-dihydroxybenzoate, gentisate and 2, 3, 4-trihydroxybenzoate. In the second step, DHB is transferred from 2, 3-dihydroxybenzoyl-AMP onto the phosphopantetheinylated EntB (holo-EntB) to form DHB-holo-EntB. Then this product will serve in the formation of the amide bond between 2, 3-dihydroxybenzoate (DHB) and L-serine. It can also transfer adenylated salicylate to holo-EntB. Proteins EntB, EntD, EntE, and EntF form a multienzyme complex called enterobactin synthase. Monomer. EntA and EntE interact together.

EXTERNAL LINKS

entE

Enterobactin synthase component E

entE

Molecular Synopsis