FEATURES

FAMILY

class-I pyridoxal-phosphate-dependent aminotransferase family

DESCRIPTION

Also known as BACF_BACSU, bacF, ywfG. Part of the bacABCDEF operon responsible for the biosynthesis of the nonribosomally synthesized dipeptide antibiotic bacilysin, composed of L-alanine and L-anticapsin. Bacilysin is an irreversible inactivator of the glutaminase domain of glucosamine synthetase (PubMed:20052993). Catalyzes the reductive amination of the C2 ketone of tetrahydro-hydroxyphenylpyruvate (H4HPP), with L-Phe as an amino donor, to yield tetrahydrotyrosine (H4Tyr) diastereomer (PubMed:22765234). D-Phe is not an effective amino donor (PubMed:22765234). BacF associated to BacG converts 3E, 7R- and 3Z, 7R-ex-H2HPP to 2S, 4R, 7R- and 2S, 4S, 7R-H4Tyr, respectively. Given that bacilysin has the 2S, 4S stereochemistry in its anticapsin moiety, it is likely that the 2S, 4S-H4Tyr is the diastereomer used for the biosynthesis (PubMed:22765234). Homodimer.

EXTERNAL LINKS

bacF

Transaminase BacF

bacF

Molecular Synopsis